PMID:10780448

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Citation

Ishibashi, M, Nakamura, K and Yamane, K' (1999) The functional analysis of 4.5S RNA in ribosomal translocation. Nucleic Acids Symp. Ser. ':199-200

Abstract

4.5S RNA is a stable 114-nucleotide RNA of the bacterium Escherichia coli (E. coli). We found that 4.5S RNA have the ability of binding EF-G using gel mobility shift assay. Increasing in the concentration of GDP increase the binding activity of 4.5S RNA to EF-G. Based on these data, we propose that 4.5S RNA release EF-G from ribosome.

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Keywords

Base Sequence; Escherichia coli/genetics; Escherichia coli/metabolism; Kinetics; Models, Genetic; Nucleic Acid Conformation; Peptide Elongation Factor G/metabolism; RNA, Bacterial/chemistry; RNA, Bacterial/metabolism; RNA, Ribosomal/chemistry; RNA, Ribosomal/metabolism; Ribosomes/metabolism

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