PMID:10780446
| Citation |
Shida, T, Kaneda, K, Ogawa, T and Sekiguchi, J' (1999) Abasic site recognition mechanism by the Escherichia coli exonuclease III. Nucleic Acids Symp. Ser. ':195-6 |
|---|---|
| Abstract |
In order to elucidate the mechanism of AP site recognition by Echerichia coli exonuclease III (exoIII), site-directed mutagenesis of the Tyr109, the Trp212, and the Phe213 residues, which were conserved in the type II AP endonuclease from various organisms and located in the vicinity of the catalytic site, was performed. The exoIII-W212S mutant lacked any detectable AP endonuclease activity and binding ability to the duplex DNA containing an AP site, while the exoIII-Y109S and exoIII-F213W mutants retained a low level of activities (13% and 83%, respectively, compared with wild-type exoIII). This study suggests that the Trp212 is an important component for abasic site recognition by the E. coli exonuclease III. |
| Links | |
| Keywords |
Amino Acid Substitution; Apurinic Acid; Base Sequence; Binding Sites; Catalytic Domain; Cloning, Molecular; Escherichia coli/enzymology; Exodeoxyribonucleases/chemistry; Exodeoxyribonucleases/genetics; Exodeoxyribonucleases/metabolism; Mutagenesis, Site-Directed; Oligodeoxyribonucleotides/chemistry; Oligodeoxyribonucleotides/metabolism; Recombinant Proteins/chemistry; Recombinant Proteins/metabolism; Substrate Specificity |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.