PMID:10771442
| Citation |
Rowland, P, Nørager, S, Jensen, KF and Larsen, S (2000) Crystallization and preliminary X-ray studies of membrane-associated Escherichia coli dihydroorotate dehydrogenase. Acta Crystallogr. D Biol. Crystallogr. 56:659-61 |
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| Abstract |
Dihydroorotate dehydrogenases (DHODs) are flavin-containing enzymes which catalyse the conversion of (S)-dihydroorotate to orotate, the fourth step in the de novo biosynthesis of pyrimidine nucleotides. Two major families of DHODs have now been identified based on their amino-acid sequence similarities. The two families differ in their reaction mechanisms, but structures are only known of enzymes belonging to family 1. DHOD from Escherichia coli is a typical member of family 2, which contains the membrane-associated enzymes from Gram-negative bacteria and eukaryotes. Yellow crystals grown of this enzyme belong to the space group P4(1)2(1)2 or P4(3)2(1)2. The unit-cell parameters are a = b = 119.2, c = 294.3 A. Owing to the rather large c axis, the currently available resolution of data is 2.2 A. |
| Links | |
| Keywords |
Cell Membrane/enzymology; Crystallization; Crystallography, X-Ray/methods; Escherichia coli/enzymology; Macromolecular Substances; Oxidoreductases/chemistry; Oxidoreductases/isolation & purification; Oxidoreductases Acting on CH-CH Group Donors |
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