PMID:10766824
| Citation |
Meibom, KL, Kallipolitis, BH, Ebright, RH and Valentin-Hansen, P (2000) Identification of the subunit of cAMP receptor protein (CRP) that functionally interacts with CytR in CRP-CytR-mediated transcriptional repression. J. Biol. Chem. 275:11951-6 |
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| Abstract |
At promoters of the Escherichia coli CytR regulon, the cAMP receptor protein (CRP) interacts with the repressor CytR to form transcriptionally inactive CRP-CytR-promoter or (CRP)(2)-CytR-promoter complexes. Here, using "oriented heterodimer" analysis, we show that only one subunit of the CRP dimer, the subunit proximal to CytR, functionally interacts with CytR in CRP-CytR-promoter and (CRP)(2)-CytR-promoter complexes. Our results provide information about the architecture of CRP-CytR-promoter and (CRP)(2)-CytR-promoter complexes and rule out the proposal that masking of activating region 2 of CRP is responsible for the transcriptional inactivity of the complexes. |
| Links | |
| Keywords |
Base Sequence; Cyclic AMP Receptor Protein/metabolism; Escherichia coli; Escherichia coli Proteins; Models, Molecular; Molecular Sequence Data; Promoter Regions, Genetic; Protein Conformation; Repressor Proteins/metabolism; Transcription, Genetic |
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