PMID:10760142
| Citation |
Weinstein-Fischer, D, Elgrably-Weiss, M and Altuvia, S (2000) Escherichia coli response to hydrogen peroxide: a role for DNA supercoiling, topoisomerase I and Fis. Mol. Microbiol. 35:1413-20 |
|---|---|
| Abstract |
Bacterial cells respond to the deleterious effects of reactive oxygen species by inducing the expression of antioxidant defence genes. Here we show that treatment with hydrogen peroxide leads to a transient decrease in DNA negative supercoiling. We also report that hydrogen peroxide activates topA P1 promoter expression. The peroxide-dependent topA P1 activation is independent of oxyR, but is mediated by Fis. This nucleoid-associated protein binds to the promoter region of topA. We also show that a fis deficient mutant strain is extremely sensitive to hydrogen peroxide. Our results suggest that topA activation by Fis is an important component of the Escherichia coli response to oxidative stress. |
| Links | |
| Keywords |
Base Sequence; Carrier Proteins/drug effects; Carrier Proteins/genetics; Carrier Proteins/metabolism; DNA Repair/drug effects; DNA Topoisomerases, Type I/drug effects; DNA Topoisomerases, Type I/genetics; DNA Topoisomerases, Type I/metabolism; DNA, Bacterial/drug effects; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/physiology; Escherichia coli Proteins; Factor For Inversion Stimulation Protein; Hydrogen Peroxide/pharmacology; Integration Host Factors; Microbial Sensitivity Tests; Molecular Sequence Data; Mutation; Oxidative Stress; Promoter Regions, Genetic; Transcription, Genetic |
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