PMID:10756520
| Citation |
Wolska, KI, Lobacz, B, Jurkiewicz, D, Bugajska, E, Kuć, M and Jóźwik, A (2000) Biosynthesis and secretion of several enzymes in Escherichia coli dnaK and dnaJ mutants. Microbios 101:157-68 |
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| Abstract |
Escherichia coli null dnaJ and dnaKdnaJ mutants were defective in the biosynthesis and secretion of several enzymes. The synthesis of beta-galactosidase induced in delta dnaJ and delta dnaKdnaJ mutants was abolished at 42 degrees C and significantly decreased at 30 and 37 degrees C. The activity of alkaline phosphatase in the periplasm in both mutant strains at high temperature was lower than in the wild-type strain. The synthesis of b-type cytochromes was defective in two deletion mutants while the synthesis of nitrate reductase-A at 42 degrees C was influenced by dnaK mutation only. The lack of DnaK and DnaJ does not impair the activity of catechol 2,3-dioxygenase irrespective of growth temperature. |
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| Keywords |
Alkaline Phosphatase/metabolism; Bacterial Proteins/biosynthesis; Bacterial Proteins/secretion; Catechol 2,3-Dioxygenase; Cytochrome b Group/biosynthesis; Cytochrome b Group/secretion; Dioxygenases; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli Proteins; Gene Deletion; HSP40 Heat-Shock Proteins; HSP70 Heat-Shock Proteins/genetics; Heat-Shock Proteins/genetics; Heating; Molecular Chaperones/genetics; Nitrate Reductase; Nitrate Reductases/biosynthesis; Nitrate Reductases/secretion; Oxygenases/metabolism; beta-Galactosidase/biosynthesis; beta-Galactosidase/secretion |
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