PMID:10754234
| Citation |
Goodrich-Blair, H and Kolter, R (2000) Homocysteine thiolactone is a positive effector of sigma(S) levels in Escherichia coli. FEMS Microbiol. Lett. 185:117-21 |
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| Abstract |
sigma(S) is a regulator of the stationary phase response in Escherichia coli. Multi-copy suppressors were sought in a strain with decreased levels of sigma(S) and one such suppressor was found to encode HsrA, a putative efflux pump. Multi-copy expression of hsrA was shown to lead to accumulation of homocysteine, which is predicted to cause an increase in homocysteine thiolactone (HCTL) levels. A direct correlation between HCTL levels and sigma(S) accumulation was observed both in mutants and during normal cell growth, leading to the hypothesis that HCTL is a physiologically relevant positive effector of sigma(S) levels in vivo. |
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| Keywords |
Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Culture Media; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Homocysteine/analogs & derivatives; Homocysteine/metabolism; Methionine/metabolism; Repressor Proteins; Sigma Factor/genetics; Sigma Factor/metabolism; Vitamin B 12/metabolism |
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