PMID:10747795
| Citation |
Fromant, M, Plateau, P and Blanquet, S (2000) Function of the extra 5'-phosphate carried by histidine tRNA. Biochemistry 39:4062-7 |
|---|---|
| Abstract |
Among elongator tRNAs, tRNA specific for histidine has the peculiarity to possess one extra nucleotide at position -1. This nucleotide is believed to be responsible for recognition by histidyl-tRNA synthetase. Here, we show that, in fact, it is the phosphate 5' to the extra nucleotide which mainly supports the efficiency of the tRNA aminoacylation reaction catalyzed by Escherichia coli histidyl-tRNA synthetase. In the case of the reaction of E. coli peptidyl-tRNA hydrolase, this atypical phosphate is dispensable. Instead, peptidyl-tRNA hydrolase recognizes the phosphate of the phosphodiester bond between residues -1 and +1 of tRNA(His). Recognition of the +1 phosphate of tRNA(His) by peptidyl-tRNA hydrolase resembles, therefore, that of the 5'-terminal phosphate of other elongator tRNAs. |
| Links | |
| Keywords |
Animals; Binding Sites; Escherichia coli; Histidine-tRNA Ligase/chemistry; Histidine-tRNA Ligase/metabolism; Phosphates; RNA, Transfer, His/chemistry; RNA, Transfer, His/genetics; RNA, Transfer, His/metabolism; Structure-Activity Relationship; Substrate Specificity |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.