PMID:10731409
| Citation |
Churchill, JJ and Kowalczykowski, SC (2000) Identification of the RecA protein-loading domain of RecBCD enzyme. J. Mol. Biol. 297:537-42 |
|---|---|
| Abstract |
Genetic recombination in Escherichia coli is stimulated by the recombination hotspot Chi (chi), a regulatory element that modifies the activities of the RecBCD enzyme and leads to loading of the DNA strand exchange protein, RecA, onto the chi-containing DNA strand. The RecBC enzyme, which lacks the RecD subunit, loads RecA protein constitutively, in a manner that is independent of chi. Using a truncated RecBC enzyme lacking the 30 kDa C-terminal domain of the RecB subunit, we show that this domain is necessary for RecA protein-loading. We propose that this domain harbors a site that interacts with RecA protein, recruiting it to single-stranded DNA during unwinding. This ability of a translocating enzyme to deliver material (RecA protein) to a specific target site (the chi sequence) parallels that of other cellular motor proteins. |
| Links |
PubMed Online version:10.1006/jmbi.2000.3590 |
| Keywords |
Base Pairing/genetics; DNA Helicases/chemistry; DNA Helicases/genetics; DNA Helicases/metabolism; DNA, Single-Stranded/chemistry; DNA, Single-Stranded/genetics; DNA, Single-Stranded/metabolism; DNA, Superhelical/chemistry; DNA, Superhelical/genetics; DNA, Superhelical/metabolism; DNA-Binding Proteins/chemistry; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Escherichia coli/enzymology; Escherichia coli Proteins; Exodeoxyribonuclease V; Exodeoxyribonucleases/chemistry; Exodeoxyribonucleases/genetics; Exodeoxyribonucleases/metabolism; Holoenzymes/chemistry; Holoenzymes/genetics; Holoenzymes/metabolism; Models, Biological; Protein Binding; Rec A Recombinases/metabolism; Recombination, Genetic/genetics; Regulatory Sequences, Nucleic Acid/genetics; Sequence Deletion/genetics |
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