PMID:10715013
| Citation |
Chen, Q, Postma, PW and Amster-Choder, O (2000) Dephosphorylation of the Escherichia coli transcriptional antiterminator BglG by the sugar sensor BglF is the reversal of its phosphorylation. J. Bacteriol. 182:2033-6 |
|---|---|
| Abstract |
The Escherichia coli BglF protein catalyzes transport and phosphorylation of beta-glucosides. In addition, BglF is a membrane sensor which reversibly phosphorylates the transcriptional regulator BglG, depending on beta-glucoside availability. Therefore, BglF has three enzymatic activities: beta-glucoside phosphotransferase, BglG phosphorylase, and phospho-BglG (BglG-P) dephosphorylase. Cys-24 of BglF is the active site which delivers the phosphoryl group either to the sugar or to BglG. To characterize the dephosphorylase activity, we asked whether BglG-P can give the phosphoryl group back to Cys-24 of BglF. Here we provide evidence which is consistent with the interpretation that Cys-24-P is an intermediate in the BglG-P dephosphorylation reaction. Hence, the dephosphorylation reaction catalyzed by BglF proceeds via reversal of the phosphorylation reaction. |
| Links | |
| Keywords |
Bacterial Proteins/metabolism; Binding Sites; Cysteine/genetics; Cysteine/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Membrane Proteins/chemistry; Membrane Proteins/genetics; Membrane Proteins/metabolism; Methylglucosides/metabolism; Models, Chemical; Mutation/genetics; Phosphorylases/metabolism; Phosphorylation; Protein Kinases/chemistry; Protein Kinases/genetics; Protein Kinases/metabolism; RNA-Binding Proteins/metabolism; Recombinant Fusion Proteins/chemistry; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.