PMID:10713426
| Citation |
Beard, SJ, Hashim, R, Wu, G, Binet, MR, Hughes, MN and Poole, RK (2000) Evidence for the transport of zinc(II) ions via the pit inorganic phosphate transport system in Escherichia coli. FEMS Microbiol. Lett. 184:231-5 |
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| Abstract |
A locus involved in zinc(II) uptake in Escherichia coli K-12 was identified through the generation of a zinc(II)-resistant mutant by transposon (Tn10dCam) mutagenesis. The mutation was located within the pitA gene, which encodes the low-affinity inorganic phosphate transport system (Pit). The pitA mutant accumulated reduced amounts of zinc(II) when exposed to 0.5-2.0 mM ZnSO(4) during growth in Luria-Bertani medium. |
| Links | |
| Keywords |
Carrier Proteins/genetics; Carrier Proteins/metabolism; Chromosome Mapping; DNA Transposable Elements; Drug Resistance, Microbial/genetics; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/metabolism; Ion Transport; Mutagenesis, Insertional; Phosphates/metabolism; Zinc/metabolism; Zinc/pharmacology |
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