PMID:10704215

Citation	Barends, S, Wower, J and Kraal, B (2000) Kinetic parameters for tmRNA binding to alanyl-tRNA synthetase and elongation factor Tu from Escherichia coli. Biochemistry 39:2652-8
Abstract	Aminoacylation and transportation of tmRNA to stalled ribosomes constitute prerequisite steps for trans-translation, a process facilitating the release of stalled ribosomes from 3' ends of truncated mRNAs and the degradation of incompletely synthesized proteins. Kinetic analysis of the aminoacylation of tmRNA indicates that tmRNA has both a lower affinity and a lower turnover number than cognate tRNA(Ala) for alanyl-tRNA synthetase, resulting in a 75-fold lower k(cat)/K(M) value. The association rate constant of Ala-tmRNA for elongation factor Tu in complex with GTP is about 150-fold lower than that of Ala-tRNA(Ala), whereas its dissocation rate constant is about 5-fold lower. These observations can be interpreted to suggest that additional factors facilitate tmRNA binding to ribosomes.
Links	PubMed
Keywords	Acylation; Alanine-tRNA Ligase/genetics; Alanine-tRNA Ligase/metabolism; Biopolymers/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/metabolism; Kinetics; Peptide Elongation Factor Tu/genetics; Peptide Elongation Factor Tu/metabolism; RNA, Messenger/metabolism; RNA, Transfer, Ala/genetics; RNA, Transfer, Ala/metabolism; Thermodynamics; Transcription, Genetic
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