PMID:10660570
| Citation |
Nurse, P, Bahng, S, Mossessova, E and Marians, KJ (2000) Mutational analysis of Escherichia coli topoisomerase IV. II. ATPase negative mutants of parE induce hyper-DNA cleavage. J. Biol. Chem. 275:4104-11 |
|---|---|
| Abstract |
ParE is the ATP-binding subunit of topoisomerase IV (Topo IV). During topoisomerization, the ATP-binding and hydrolysis cycle must be coordinated with the cycle of DNA cleavage and religation. We have isolated three dominant-negative mutant alleles of parE that encode ParE proteins that fail to hydrolyze ATP when reconstituted with ParC to form Topo IV. ParE G110S Topo IV and ParE S123L Topo IV failed to bind ATP at all, whereas ParE T201A could bind ATP. All three mutant Topo IV proteins exhibited an elevated level of spontaneous DNA cleavage that could be associated with a decreased rate of DNA resealing. In ParE T201A Topo IV, this defect appeared to result from an increased likelihood that the tetrameric enzyme would fall apart after DNA cleavage. Thus, while ATP is not required for DNA cleavage, the properties of these mutant enzymes suggests that ATP-hydrolysis informs DNA religation. |
| Links | |
| Keywords |
Adenosine Triphosphatases/genetics; Adenosine Triphosphate/metabolism; Adenylyl Imidodiphosphate/metabolism; Animals; Bacterial Proteins/genetics; Chromatography, Gel; Crithidia fasciculata; DNA Mutational Analysis; DNA Topoisomerase IV; DNA Topoisomerases, Type II/genetics; DNA, Superhelical/metabolism; DNA-Binding Proteins/genetics; Dimerization; Escherichia coli/enzymology; Models, Molecular; Mutation |
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