PMID:10656793
| Citation |
Janosi, L, Mori, H, Sekine, Y, Abragan, J, Janosi, R, Hirokawa, G and Kaji, A (2000) Mutations influencing the frr gene coding for ribosome recycling factor (RRF). J. Mol. Biol. 295:815-29 |
|---|---|
| Abstract |
A total of 52 null, six reversion, and five silent mutations of frr (the gene encoding for ribosome recycling factor (RRF)) of Escherichia coli are discussed along with 12 temperature-sensitive (ts) mutations and 14 intergenic suppressor strains of ts RRF. The null mutations were classified into six different categories. A computer-based secondary structure analysis showed three domains; domain A which has the N-terminal helix, domain B which contains coil, alpha-helix and beta-strand structure, and domain C which is a C-terminal helix. The ts mutations fell into domains A and C but not in domain B. More than a half of the null mutations fell into domain B while the silent mutations fell outside domain B. Substitution of Arg132 in domain C by other amino acids was observed among five independently isolated null mutants. It is suggested that domain B is important for maintaining the RRF structure, while the region including Arg132 is one of the active sites. A total of 14 intergenic suppressor strains of ts RRF were grouped into four categories, depending on which temperature-sensitive alleles were suppressed. |
| Links |
PubMed Online version:10.1006/jmbi.1999.3401 |
| Keywords |
Amino Acid Sequence; Amino Acid Substitution; Escherichia coli/genetics; Escherichia coli/metabolism; Genes, Bacterial; Introns; Molecular Sequence Data; Mutagenesis, Site-Directed; Proteins/chemistry; Proteins/genetics; Proteins/metabolism; Recombinant Proteins/chemistry; Recombinant Proteins/metabolism; Ribosomal Proteins; Ribosomes/genetics; Ribosomes/metabolism; Suppression, Genetic; Temperature; Thermodynamics |
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