PMID:10620686
| Citation |
Stentebjerg-Olesen, B, Chakraborty, T and Klemm, P (2000) FimE-catalyzed off-to-on inversion of the type 1 fimbrial phase switch and insertion sequence recruitment in an Escherichia coli K-12 fimB strain. FEMS Microbiol. Lett. 182:319-25 |
|---|---|
| Abstract |
We have investigated the capacity of a well-defined Escherichia coli fimB strain, AAEC350 (a derivative of MG1655), to express type 1 fimbriae under various growth conditions. The expression of type 1 fimbriae is phase-variable due to the inversion of a 314-bp DNA segment. Two tyrosine recombinases, FimB and FimE, mediate the inversion of the phase switch. FimB can carry out recombination in both directions, whereas the current evidence suggests that FimE-catalyzed switching is on-to-off only. We show here that AAEC350 is in fact capable of off-to-on phase switching and type 1 fimbrial expression under aerobic static growth conditions. The phase switching is mediated by FimE, and allows emerging fimbriate AAEC350 to outgrow their non-fimbriate counterparts by pellicle formation. Following inversion of the phase switch, this element can remain phase-locked in the on orientation due to integration of insertion sequence elements, viz. IS1 or IS5, at various positions in either the fimE gene or the phase switch. |
| Links | |
| Keywords |
Agglutination; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Culture Media; DNA Transposable Elements; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Escherichia coli/ultrastructure; Escherichia coli Proteins; Fimbriae, Bacterial/metabolism; Gene Expression Regulation, Bacterial; Integrases; Polymerase Chain Reaction/methods; Recombination, Genetic; Sequence Analysis, DNA |
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