PMID:10602715
| Citation |
Oethinger, M, Kern, WV, Jellen-Ritter, AS, McMurry, LM and Levy, SB (2000) Ineffectiveness of topoisomerase mutations in mediating clinically significant fluoroquinolone resistance in Escherichia coli in the absence of the AcrAB efflux pump. Antimicrob. Agents Chemother. 44:10-3 |
|---|---|
| Abstract |
Fluoroquinolone-resistant mutants, selected from a wild-type Escherichia coli K-12 strain and its Mar mutant by exposure to increasing levels of ofloxacin on solid medium, were analyzed by Northern (RNA) blot analysis, sequencing, and radiolabelled ciprofloxacin accumulation studies. Mutations in the target gene gyrA (DNA gyrase), the regulatory gene marR, and additional, as yet unidentified genes (genes that probably affect efflux mediated by the multidrug efflux pump AcrAB) all contributed to fluoroquinolone resistance. Inactivation of the acrAB locus made all strains, including those with target gene mutations, hypersusceptible to fluoroquinolones and certain other unrelated drugs. These studies indicate that, in the absence of the AcrAB pump, gyrase mutations fail to produce clinically relevant levels of fluoroquinolone resistance. |
| Links | |
| Keywords |
Anti-Infective Agents/pharmacology; Bacterial Proteins/physiology; Ciprofloxacin/pharmacokinetics; DNA Gyrase; DNA Topoisomerases, Type II/genetics; DNA Topoisomerases, Type II/physiology; Drug Resistance, Microbial; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli Proteins; Gene Deletion; Genes, Bacterial; Genes, Regulator; Humans; Lipoproteins/physiology; Membrane Transport Proteins; Mutation |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<protect><annotationlinks/></protect>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.
