PMID:10595554

Citation	Chivers, PT and Sauer, RT (1999) NikR is a ribbon-helix-helix DNA-binding protein. Protein Sci. 8:2494-500
Abstract	Escherichia coli NikR, a repressor with homologs in other bacteria and archaea, was identified as a potential new member of the ribbon-helix-helix (beta-alpha-alpha) family of transcription factors in profile based sequence searches and in structure prediction experiments. Biophysical and biochemical characterization of the N-terminal domain of NikR show that it has many features expected of a beta-alpha-alpha protein including alpha-helical content, dimeric solution form, concentration dependent thermal stability, and ability to bind DNA in sequence-specific manner. Mutation of a residue predicted to be important for DNA-binding reduces operator affinity but does not affect the secondary structure or stability of the protein.
Links	PubMed PMC2144182 Online version:10.1110/ps.8.11.2494
Keywords	Amino Acid Sequence; Base Sequence; Calorimetry; Circular Dichroism; Cloning, Molecular; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Models, Molecular; Molecular Sequence Data; Operon; Promoter Regions, Genetic; Protein Structure, Secondary; Recombinant Proteins/chemistry; Repressor Proteins/chemistry; Repressor Proteins/genetics; Sequence Alignment; Sequence Homology, Amino Acid
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