PMID:10572959
| Citation |
Lyngstadaas, A, Løbner-Olesen, A, Grelland, E and Boye, E (1999) The gene for 2-phosphoglycolate phosphatase (gph) in Escherichia coli is located in the same operon as dam and at least five other diverse genes. Biochim. Biophys. Acta 1472:376-84 |
|---|---|
| Abstract |
Downstream of the dam gene in the Escherichia coli genome the following three genes are located: first rpe, then a gene encoding a 27 kDa protein and finally trpS. Here we present evidence that the 27 kDa protein has 2-phosphoglycolate phosphatase activity, and we name the gene gph. Phosphoglycolate phosphatase is needed in autotrophic organisms performing the Calvin-Benson-Bassham (CBB) reductive pentose-phosphate cycle. E. coli is not capable of autotrophic growth and probably utilizes Gph activity for other function(s) than in the CBB cycle. We found no physiological effect of deleting gph and its function in E. coli remains unclear. The use of fusion plasmids, where lacZ was inserted into gph and trpS, and deletion derivatives of these fusion plasmids, showed that rpe, gph and trpS are all members of the dam-containing operon. A novel promoter was identified in the distal part of the dam gene. The operon, which contains aroK, aroB, urf74.3, dam, rpe, gph, and trpS, can be termed a superoperon, since it consists of (at least) seven apparently unrelated genes which are under complex regulatory control. |
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| Keywords |
Artificial Gene Fusion; Base Sequence; DNA, Bacterial; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli Proteins; Genes, Bacterial; Lac Operon; Molecular Sequence Data; Operon; Phosphoric Monoester Hydrolases/genetics; Plasmids; Promoter Regions, Genetic; Sequence Deletion; Site-Specific DNA-Methyltransferase (Adenine-Specific)/genetics; Transcription, Genetic |
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