PMID:10559180
| Citation |
Poulsen, TS, Chang, YY and Hove-Jensen, B (1999) D-Allose catabolism of Escherichia coli: involvement of alsI and regulation of als regulon expression by allose and ribose. J. Bacteriol. 181:7126-30 |
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| Abstract |
Genes involved in allose utilization of Escherichia coli K-12 are organized in at least two operons, alsRBACE and alsI, located next to each other on the chromosome but divergently transcribed. Mutants defective in alsI (allose 6-phosphate isomerase gene) and alsE (allulose 6-phosphate epimerase gene) were Als(-). Transcription of the two allose operons, measured as beta-galactosidase activity specified by alsI-lacZ(+) or alsE-lacZ(+) operon fusions, was induced by allose. Ribose also caused derepression of expression of the regulon under conditions in which ribose phosphate catabolism was impaired. |
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| Keywords |
Base Sequence; Escherichia coli/enzymology; Escherichia coli/genetics; Gene Expression Regulation, Bacterial; Glucose/metabolism; Isomerases/genetics; Isomerases/metabolism; Molecular Sequence Data; Operon/genetics; Racemases and Epimerases/genetics; Regulon; Ribose/metabolism; Transcription, Genetic |
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