PMID:10557284
| Citation |
Yin, H, Artsimovitch, I, Landick, R and Gelles, J (1999) Nonequilibrium mechanism of transcription termination from observations of single RNA polymerase molecules. Proc. Natl. Acad. Sci. U.S.A. 96:13124-9 |
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| Abstract |
Cessation of transcription at specific terminator DNA sequences is used by viruses, bacteria, and eukaryotes to regulate the expression of downstream genes, but the mechanisms of transcription termination are poorly characterized. To elucidate the kinetic mechanism of termination at the intrinsic terminators of enteric bacteria, we observed, by using single-molecule light microscopy techniques, the behavior of surface-immobilized Escherichia coli RNA polymerase (RNAP) molecules in vitro. An RNAP molecule remains at a canonical intrinsic terminator for approximately 64 s before releasing DNA, implying the formation of an elongation-incompetent (paused) intermediate by transcription complexes that terminate but not by those that read through the terminator. Analysis of pause lifetimes establishes a complete minimal mechanism of termination in which paused intermediate formation is both necessary and sufficient to induce release of RNAP at the terminator. The data suggest that intrinsic terminators function by a nonequilibrium process in which terminator effectiveness is determined by the relative rates of nucleotide addition and paused state entry by the transcription complex. |
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| Keywords |
Base Sequence; DNA/chemistry; DNA Primers; DNA-Directed RNA Polymerases/chemistry; DNA-Directed RNA Polymerases/genetics; Nucleic Acid Conformation; Protein Structure, Secondary; Terminator Regions, Genetic; Transcription, Genetic/genetics |
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