PMID:10550691
| Citation |
Mulliez, E, Ollagnier-de Choudens, S, Meier, C, Cremonini, M, Luchinat, C, Trautwein, AX and Fontecave, M (1999) Iron-sulfur interconversions in the anaerobic ribonucleotide reductase from Escherichia coli. J. Biol. Inorg. Chem. 4:614-20 |
|---|---|
| Abstract |
The anaerobic ribonucleotide reductase from Escherichia coli contains an iron-sulfur cluster which, in the reduced [4Fe-4S](+) form, serves to reduce S-adenosylmethionine and to generate a catalytically essential glycyl radical. The reaction of the reduced cluster with oxygen was studied by UV-visible, EPR, NMR, and Mössbauer spectroscopies. The [4Fe-4S](+) form is shown to be extremely sensitive to oxygen and converted to [4Fe-4S](2+), [3Fe-4S](+/0), and to the stable [2Fe-2S](2+) form. It is remarkable that the oxidized protein retains full activity. This is probably due to the fact that during reduction, required for activity, the iron atoms, from 2Fe and 3Fe clusters, readily reassemble to generate an active [4Fe-4S] center. This property is discussed as a possible protective mechanism of the enzyme during transient exposure to air. Furthermore, the [2Fe-2S] form of the protein can be converted into a [3Fe-4S] form during chromatography on dATP-Sepharose, explaining why previous preparations of the enzyme were shown to contain large amounts of such a 3Fe cluster. This is the first report of a 2Fe to 3Fe cluster conversion. |
| Links | |
| Keywords |
Anaerobiosis; Electron Spin Resonance Spectroscopy; Escherichia coli/enzymology; Iron/metabolism; Magnetic Resonance Spectroscopy; Oxidation-Reduction; Ribonucleotide Reductases/chemistry; Ribonucleotide Reductases/metabolism; Spectroscopy, Mossbauer; Sulfur/metabolism |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.