PMID:10548535
| Citation |
Potter, LC and Cole, JA (1999) Essential roles for the products of the napABCD genes, but not napFGH, in periplasmic nitrate reduction by Escherichia coli K-12. Biochem. J. 344 Pt 1:69-76 |
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| Abstract |
The seven nap genes at minute 47 on the Escherichia coli K-12 chromosome encode a functional nitrate reductase located in the periplasm. The molybdoprotein, NapA, is known to be essential for nitrate reduction. We now demonstrate that the two c-type cytochromes, the periplasmic NapB and the membrane-associated NapC, as well as a fourth polypeptide, NapD, are also essential for nitrate reduction in the periplasm by physiological substrates such as glycerol, formate and glucose. None of the three iron-sulphur proteins, NapF, NapG or NapH, are essential, irrespective of whether the bacteria are grown anaerobically in the presence of nitrate or fumarate as a terminal electron acceptor, or by glucose fermentation. Mutation of napD resulted in the total loss of Methyl Viologen-dependent nitrate reductase activity of the molybdoprotein, NapA, consistent with an earlier suggestion by others that NapD might be required for post-translational modification of NapA. |
| Links | |
| Keywords |
Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Cytochrome c Group/genetics; Cytochrome c Group/metabolism; DNA Primers/genetics; Electron Transport; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Escherichia coli Proteins; Fermentation; Fumarates/metabolism; Gene Deletion; Genes, Bacterial; Glucose/metabolism; Mutation; Nitrate Reductase; Nitrate Reductases/genetics; Nitrate Reductases/metabolism; Nitric Acid/metabolism; Oxidation-Reduction; Periplasm/metabolism; Plasmids/genetics |
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