PMID:10531489
| Citation |
Kobori, T, Lee, WC, Akagi, T, Sasaki, H, Zenno, S, Saigo, K and Tanokura, M (1999) Crystallization and preliminary crystallographic analysis of major nitroreductase from Escherichia coli. Acta Crystallogr. D Biol. Crystallogr. 55:1901-2 |
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| Abstract |
NADPH:nitrocompound oxidoreductase from Escherichia coli, NfsA, has been crystallized in the presence of FMN by the vapor-diffusion method using polyethylene glycol 6000 as a precipitant. The crystals belonged to the triclinic space group P1 with cell dimensions, a = 52.2, b = 52.7, c = 53.3 A, alpha = 75.1, beta = 60.1, gamma = 60.5 degrees. The crystals are expected to contain two NfsA molecules per asymmetric unit. The crystals diffracted X-rays to at least 2.3 A resolution and are appropriate for structural analysis at high resolution. |
| Links | |
| Keywords |
Crystallization; Crystallography, X-Ray; Drug Resistance, Neoplasm; Escherichia coli; Escherichia coli Proteins; Flavin Mononucleotide/chemistry; NADP/chemistry; Nitroreductases/chemistry; Polyethylene Glycols; Software |
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