PMID:10521694
| Citation |
Takimoto, K, Tano, K, Hashimoto, M, Hori, M, Akasaka, S and Utsumi, H (1999) Delayed transfection of DNA after riboflavin mediated photosensitization increases G:C to C:G transversions of supF gene in Escherichia coli mutY strain. Mutat. Res. 445:93-8 |
|---|---|
| Abstract |
We have previously reported that the majority of base substitution mutations of the Escherichia coli supF gene induced by riboflavin mediated photosensitization were G:C to C:G changes, in addition to G:C to T:A changes which were probably caused by 8-hydroxyguanine (oh(8)Gua), in wild type and mutM mutator mutant strains. This implies that lesions other than oh(8)Gua are produced by riboflavin-photosensitization. G:C to C:G base substitutions have been found in the mutations induced by ionizing radiation and reactive oxygen species, as well as spontaneous mutation. To characterize the G:C to C:G mutation, riboflavin- photosensitized plasmid DNA carrying the supF gene was left at room temperature for 5 h in the dark before transfection. The delayed transfection gave a mutational spectrum different from that for immediate transfection. G:C to C:G transversions significantly increased in mutY mutator strain, in which the transversion was not detected in the immediate transfection. Lesions causing G:C to C:G changes increased during 5-h holding after photosensitization and MutY protein presumably takes part in this type of base change mutation. |
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| Keywords |
Base Sequence; DNA Glycosylases; DNA Mutational Analysis; DNA, Bacterial/chemistry; DNA, Bacterial/drug effects; DNA, Bacterial/radiation effects; Escherichia coli/genetics; Genes, Suppressor; Molecular Sequence Data; Mutation; N-Glycosyl Hydrolases/genetics; Photosensitizing Agents/pharmacology; Plasmids/drug effects; Plasmids/genetics; Plasmids/radiation effects; Point Mutation; RNA, Transfer/genetics; Riboflavin/pharmacology; Time Factors; Transformation, Bacterial |
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