PMID:10521403
| Citation |
Coburn, GA, Miao, X, Briant, DJ and Mackie, GA (1999) Reconstitution of a minimal RNA degradosome demonstrates functional coordination between a 3' exonuclease and a DEAD-box RNA helicase. Genes Dev. 13:2594-603 |
|---|---|
| Abstract |
The RNA degradosome is a multiprotein complex required for the degradation of highly structured RNAs. We have developed a method for reconstituting a minimal degradosome from purified proteins. Our results demonstrate that a degradosome-like complex containing RNase E, PNPase, and RhlB can form spontaneously in vitro in the absence of all other cellular components. Moreover, ATP-dependent degradation of the malEF REP RNA by the reconstituted, minimal degradosome is indistinguishable from that of degradosomes isolated from whole cells. The Rne protein serves as an essential scaffold in the reconstitution process; however, RNase E activity is not required. Rather, Rne coordinates the activation of RhlB dependent on a 3' single-stranded extension on RNA substrates. A model for degradosome-mediated degradation of structured RNA is presented with its implications for mRNA decay in Escherichia coli. |
| Links | |
| Keywords |
ATP-Binding Cassette Transporters; Base Sequence; Carrier Proteins/metabolism; Endoribonucleases/metabolism; Endoribonucleases/physiology; Escherichia coli/genetics; Escherichia coli Proteins; Exonucleases/metabolism; Exonucleases/physiology; Maltose-Binding Proteins; Molecular Sequence Data; Monosaccharide Transport Proteins; Multienzyme Complexes/metabolism; Multienzyme Complexes/physiology; Nucleic Acid Conformation; Polyribonucleotide Nucleotidyltransferase/metabolism; Polyribonucleotide Nucleotidyltransferase/physiology; RNA Helicases/metabolism; RNA Helicases/physiology; RNA, Bacterial/chemistry |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.