PMID:10497017
| Citation |
Wang, WF, Margolin, W and Molineux, IJ (1999) Increased synthesis of an Escherichia coli membrane protein suppresses F exclusion of bacteriophage T7. J. Mol. Biol. 292:501-12 |
|---|---|
| Abstract |
Increased synthesis of the protein FxsA alleviates the exclusion of T7 in cells harboring the F plasmid. In contrast to wild-type or cells defective in fxsA, overexpression of fxsA+ allows T7 to form plaques at normal efficiency even though the burst size is reduced to about half that obtained on the isogenic F- strain. No defect in DNA synthesis was observed but late protein synthesis remains partially inhibited and a reduced level of cell leakiness, a prominent feature of F+ cells abortively infected by T7, persists. The FxsA protein is shown to be a cytoplasmic membrane protein. How T7 avoids exclusion by F in cells that exhibit increased levels of FxsA is discussed in terms of its membrane localization. |
| Links |
PubMed Online version:10.1006/jmbi.1999.3088 |
| Keywords |
Amino Acid Sequence; Bacterial Proteins/biosynthesis; Bacterial Proteins/genetics; Bacteriophage T7/genetics; DNA Replication; DNA, Bacterial/analysis; Escherichia coli/genetics; Escherichia coli/virology; Escherichia coli Proteins; F Factor/genetics; Gene Expression Regulation, Bacterial; Membrane Proteins/biosynthesis; Microscopy, Fluorescence; Molecular Sequence Data; Mutation; Nucleotides/analysis; Recombinant Fusion Proteins; Suppression, Genetic |
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