PMID:10488344
| Citation |
Wagner, J, Gruz, P, Kim, SR, Yamada, M, Matsui, K, Fuchs, RP and Nohmi, T (1999) The dinB gene encodes a novel E. coli DNA polymerase, DNA pol IV, involved in mutagenesis. Mol. Cell 4:281-6 |
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| Abstract |
In Escherichia coli, the dinB gene is required for the SOS-induced lambda untargeted mutagenesis pathway and confers a mutator phenotype to the cell when the gene product is overexpressed. Here, we report that the purified DinB protein is a DNA polymerase. This novel E. coli DNA polymerase (pol IV) is shown to be strictly distributive, devoid of proofreading activity, and prone to elongate bulged (misaligned) primer/template structures. Site-directed mutagenesis experiments of dinB also demonstrate that the polymerase activity of DinB is required for its in vivo mutagenicity. Along with the sequence homologies previously found within the UmuC-like protein family, these results indicate that the uncovered DNA polymerase activity may be a common feature of all these homologous proteins. |
| Links | |
| Keywords |
Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; DNA Polymerase beta/genetics; DNA Polymerase beta/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/radiation effects; Escherichia coli Proteins; Mutagenesis; Mutagenesis, Site-Directed; Oligodeoxyribonucleotides; Recombinant Proteins/biosynthesis; Recombinant Proteins/metabolism; SOS Response (Genetics); Substrate Specificity; Templates, Genetic; Ultraviolet Rays |
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