PMID:10483733
| Citation |
Qi, H, Menzel, R and Tse-Dinh, YC (1999) Increased thermosensitivity associated with topoisomerase I deletion and promoter mutations in Escherichia coli. FEMS Microbiol. Lett. 178:141-6 |
|---|---|
| Abstract |
An Escherichia coli mutant with three of the promoters for the topoisomerase I gene (topA) deleted, such that only the sigma 32-dependent promoter (P1) remained, had a decreased level of topoisomerase I at 30 degrees C and showed increased thermosensitivity at 52 degrees C. However, it could still develop thermotolerance and had a wild-type level of resistance to 52 degrees C treatment if exposed first to 42 degrees C. This indicated that newly synthesized topoisomerase I from transcription initiated at P1 was important for development of thermotolerance. Two other E. coli mutants lacking topA were > 100 times more sensitive to high temperature than their wild-type isogenic strains. |
| Links | |
| Keywords |
DNA Topoisomerases, Type I/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/physiology; Gene Deletion; Genes, Bacterial/genetics; Genotype; Hot Temperature; Mutation; Promoter Regions, Genetic/genetics; Sigma Factor/genetics |
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