PMID:10481090
| Citation |
Grassl, G, Bufe, B, Müller, B, Rösel, M and Kleiner, D (1999) Characterization of the gltF gene product of Escherichia coli. FEMS Microbiol. Lett. 179:79-84 |
|---|---|
| Abstract |
The glt operon of Escherichia coli comprises the structural genes for the glutamate synthase subunits (gltB and gltD) and gltF, whose product was previously suggested to have regulatory functions. The A/T-rich region between gltD and gltF contains a weak promoter and a translation initiation site for gltF. The GltF protein is preceded by a signal peptide, which is cleaved off during export into the periplasmic space. A gltF::Km(R) insertion mutant was constructed and shown here to have no detectable phenotype with respect to amino acid utilization or ammonium transport. Thus, GltF is apparently not involved in regulation of nitrogen catabolism. |
| Links | |
| Keywords |
AT Rich Sequence; Amino Acid Sequence; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Escherichia coli/genetics; Escherichia coli/physiology; Escherichia coli Proteins; Genotype; Molecular Sequence Data; Operon; Periplasmic Proteins; Phenotype; Promoter Regions, Genetic; Protein Biosynthesis; Protein Sorting Signals/genetics; Sequence Alignment; Sequence Homology, Amino Acid |
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