PMID:10480943
| Citation |
Hiasa, H and Marians, KJ (1999) Initiation of bidirectional replication at the chromosomal origin is directed by the interaction between helicase and primase. J. Biol. Chem. 274:27244-8 |
|---|---|
| Abstract |
Several protein-protein interactions have been shown to be critical for proper replication fork function in Escherichia coli. These include interactions between the polymerase and the helicase, the helicase and the primase, and the primase and the polymerase. We have studied the influence of these interactions on proper initiation at oriC by using mutant primases defective in their interaction with the helicase and DNA polymerase III holoenzyme lacking the tau subunit so that it will not interact with the helicase. We show here that accurate initiation of bidirectional DNA replication from oriC is dependent on proper placement of the primers for leading strand synthesis and is thus governed primarily by the interaction between the helicase and primase. |
| Links | |
| Keywords |
Bacterial Proteins; DNA Helicases/metabolism; DNA Primase/metabolism; DNA Replication; DNA, Bacterial/biosynthesis; DNA-Binding Proteins/biosynthesis; DNA-Binding Proteins/genetics; DnaB Helicases; Electrophoresis, Agar Gel; Escherichia coli/genetics; Origin Recognition Complex; Protein Binding; Viral Proteins/biosynthesis; Viral Proteins/genetics |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.