PMID:10471732

Citation	Zaychikov, E, Denissova, L, Guckenberger, R and Heumann, H (1999) Escherichia coli RNA polymerase translocation is accompanied by periodic bending of the DNA. Nucleic Acids Res. 27:3645-52
Abstract	RNA polymerase was halted in consecutive registers of RNA synthesis ranging from registers 11 to 68. Non-denaturing gel electrophoresis shows that the mobility of the complexes varies (up to 15%), indicating that halted complexes differ in their conformation. The electrophoretic mobility changes with an approximate 10-register periodicity. The change of the mobility can be attributed to relative changes of RNA polymerase-induced bending angle. We suggest that the periodicity of the bending angle reflects periodic changes of the conformation of the halted complexes that might have relevance for the translocation mechanism.
Links	PubMed PMC148618
Keywords	Base Sequence; Binding Sites; DNA, Bacterial/chemistry; DNA, Bacterial/genetics; DNA, Bacterial/metabolism; DNA-Directed RNA Polymerases/chemistry; DNA-Directed RNA Polymerases/metabolism; Electrophoresis, Polyacrylamide Gel; Escherichia coli/enzymology; Isomerism; Nucleic Acid Conformation; Protein Conformation; RNA, Bacterial/biosynthesis; RNA, Bacterial/genetics; Sequence Deletion; Templates, Genetic; Time Factors; Transcription, Genetic/genetics
edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Add links to pages that link here (e.g. gene, product, method pages)

See Help:References for how to manage references in EcoliWiki.