PMID:10464230

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Citation

Kato, A, Tanabe, H and Utsumi, R (1999) Molecular characterization of the PhoP-PhoQ two-component system in Escherichia coli K-12: identification of extracellular Mg2+-responsive promoters. J. Bacteriol. 181:5516-20

Abstract

We identified Mg2+-responsive promoters of the phoPQ, mgtA, and mgrB genes of Escherichia coli K-12 by S1 nuclease analysis. Expression of these genes was induced by magnesium limitation and depended on PhoP and PhoQ. The transcription start sites were also determined, which allowed us to find a (T/G)GTTTA direct repeat in their corresponding promoter regions.

Links

PubMed PMC94065

Keywords

Adenosine Triphosphatases/genetics; Bacterial Proteins/genetics; Carrier Proteins/genetics; DNA Transposable Elements; Escherichia coli/genetics; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Genes, Bacterial; Magnesium; Membrane Transport Proteins; Promoter Regions, Genetic; Signal Transduction

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