PMID:10464228
| Citation |
Raffaelli, N, Lorenzi, T, Mariani, PL, Emanuelli, M, Amici, A, Ruggieri, S and Magni, G (1999) The Escherichia coli NadR regulator is endowed with nicotinamide mononucleotide adenylyltransferase activity. J. Bacteriol. 181:5509-11 |
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| Abstract |
The first identification and characterization of a catalytic activity associated with NadR protein is reported. A computer-aided search for sequence similarity revealed the presence in NadR of a 29-residue region highly conserved among known nicotinamide mononucleotide adenylyltransferases. The Escherichia coli nadR gene was cloned into a T7-based vector and overexpressed. In addition to functionally specific DNA binding properties, the homogeneous recombinant protein catalyzes NAD synthesis from nicotinamide mononucleotide and ATP. |
| Links | |
| Keywords |
Amino Acid Sequence; Bacterial Proteins; Cloning, Molecular; DNA/metabolism; Escherichia coli; Gene Expression; Molecular Sequence Data; Nicotinamide-Nucleotide Adenylyltransferase/metabolism; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/isolation & purification; Recombinant Fusion Proteins/metabolism; Repressor Proteins/genetics; Repressor Proteins/isolation & purification; Repressor Proteins/metabolism |
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