PMID:10455182
| Citation |
Liu, J and Marians, KJ (1999) PriA-directed assembly of a primosome on D loop DNA. J. Biol. Chem. 274:25033-41 |
|---|---|
| Abstract |
Escherichia coli strains carrying null mutations in priA are chronically induced for the SOS response and are defective in homologous recombination, repair of UV damaged DNA, double-strand break repair, and both induced and constitutive stable DNA replication. This led to the proposal that PriA directed replication fork assembly at D loops formed by the homologous recombination machinery. The demonstration that PriA specifically recognized and bound D loop DNA supported this hypothesis. Using DNA footprinting as an assay, we show here that PriA also directs the assembly of a varphiX174-type primosome on D loop DNA. The ability to load a complete primosome on D loop DNA is a step necessary for replication fork assembly. |
| Links | |
| Keywords |
Adenosine Triphosphatases/genetics; Adenosine Triphosphatases/metabolism; Base Sequence; DNA/chemistry; DNA Footprinting; DNA Helicases/metabolism; DNA Replication; DNA, Single-Stranded/metabolism; DNA-Binding Proteins/metabolism; Deoxyribonuclease I; Electrophoresis, Polyacrylamide Gel; Escherichia coli Proteins; Molecular Sequence Data; Nucleic Acid Conformation; Recombination, Genetic; Single-Strand Specific DNA and RNA Endonucleases |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.
