PMID:10455032
| Citation |
Dobbin, PS, Butt, JN, Powell, AK, Reid, GA and Richardson, DJ (1999) Characterization of a flavocytochrome that is induced during the anaerobic respiration of Fe3+ by Shewanella frigidimarina NCIMB400. Biochem. J. 342 ( Pt 2):439-48 |
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| Abstract |
A 63.9 kDa periplasmic tetrahaem flavocytochrome c(3), designated Ifc(3), was found to be expressed in Shewanella frigidimarina NCIMB400 grown anaerobically with ferric citrate or ferric pyrophosphate as the sole terminal electron acceptor, but not in anaerobic cultures of the bacterium with other respiratory substrates. Ifc(3) was purified to homogeneity and revealed by biochemical, spectroscopic and primary structure analyses to contain four low-spin bis-His-ligated c(3)-haems, with midpoint reduction potentials of -73, -141, -174 and -259 mV. A low-potential flavin was present in the form of non-covalently bound FAD; the protein possessed a unidirectional fumarate reductase activity. Disruption of the chromosomal gene encoding Ifc(3), ifcA, did not lead to a significant change in the rate of Fe(3+) reduction in batch culture. However, during such growth the Ifc(3)-deficient mutant produced both a 35 kDa periplasmic c-type cytochrome and a 45 kDa membrane-associated c-type cytochrome at markedly higher levels than did the parent strain. Nucleotide sequencing data from directly upstream of ifcA indicated the presence of an open reading frame encoding a putative outer-membrane beta-barrel protein of 324 amino acid residues. |
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| Keywords |
Amino Acid Sequence; Anaerobiosis; Base Sequence; Cloning, Molecular; Cytochrome c Group/biosynthesis; Cytochrome c Group/chemistry; Cytochrome c Group/genetics; DNA, Bacterial/genetics; Electron Spin Resonance Spectroscopy; Genes, Bacterial; Iron/metabolism; Kinetics; Molecular Sequence Data; Molecular Weight; Mutation; Oxidation-Reduction; Sequence Homology, Amino Acid; Shewanella/genetics; Shewanella/growth & development; Shewanella/metabolism; Spectrophotometry; Succinate Dehydrogenase/biosynthesis; Succinate Dehydrogenase/chemistry; Succinate Dehydrogenase/genetics |
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