PMID:10441470
| Citation |
Agemizu, Y, Uematsu, N and Yamamoto, K (1999) DNA sequence analysis of spontaneous tonB deletion mutations in a polA1 strain of Escherichia coli K12. Biochem. Biophys. Res. Commun. 261:584-9 |
|---|---|
| Abstract |
By DNA sequence analysis, we have determined a spectrum of 61 spontaneous mutations occurring in the endogenous tonB gene in the polA1 strain of Escherichia coli. The overall mutation frequency was approximately 2.4-fold higher in the polA1 strain and this was attributable to enhanced rates of deletion and frameshift mutations. Among 39 deletions, a hot spot (17 mutations) was detected: a 13-bp deletion presumably directed by a 3-bp repeated sequence at its end points. The remaining 22 were distributed among 19 different mutations either flanked (16/19) or not flanked (3/19) by repeated sequences. Single-base frameshifts accounted for 8 mutations of either repeated (3/8) or nonrepeated (5/8) bases among which 6 were minus one frameshift. In contrast to previous reports, we did not frequently observe a 5'-GTGG-3' sequence in the vicinity of the deletions and frameshifts. The results presented here indicated an anti-deletion and anti-frameshift role for DNA polymerase I. |
| Links |
PubMed Online version:10.1006/bbrc.1999.1074 |
| Keywords |
Bacterial Proteins/genetics; Base Sequence; DNA Mutational Analysis; DNA Polymerase I/metabolism; Escherichia coli/genetics; Escherichia coli Proteins; Frameshift Mutation; Gene Deletion; Membrane Proteins/genetics; Repetitive Sequences, Nucleic Acid; Species Specificity |
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