PMID:10438794
| Citation |
Brooun, A, Tomashek, JJ and Lewis, K (1999) Purification and ligand binding of EmrR, a regulator of a multidrug transporter. J. Bacteriol. 181:5131-3 |
|---|---|
| Abstract |
EmrR, the repressor of the emrRAB operon of Escherichia coli, was purified to 95% homogeneity. EmrR was found to bind putative ligands of the EmrAB pump-2,4-dinitrophenol, carbonyl cyanide m-chlorophenylhydrazone, and carbonyl cyanide p-(trifluoro-methoxy)phenylhydrazone-with affinities in the micromolar range. Equilibrium dialysis experiments suggested one bound ligand per monomer of the dimeric EmrR. |
| Links | |
| Keywords |
2,4-Dinitrophenol/metabolism; 2,4-Dinitrophenol/pharmacology; Bacterial Proteins/genetics; Bacterial Proteins/isolation & purification; Bacterial Proteins/metabolism; Binding, Competitive/drug effects; Carbonyl Cyanide m-Chlorophenyl Hydrazone/metabolism; Carbonyl Cyanide m-Chlorophenyl Hydrazone/pharmacology; Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone/metabolism; Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone/pharmacology; Dialysis; Escherichia coli/chemistry; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Ligands; P-Glycoprotein/isolation & purification; P-Glycoprotein/metabolism; Repressor Proteins/genetics; Repressor Proteins/isolation & purification; Repressor Proteins/metabolism; Transcription Factors |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.