PMID:10434023

Citation	Doiron, KM, Lavigne-Nicolas, J and Cupples, CG (1999) Effect of interaction between 5-azacytidine and DNA (cytosine-5) methyltransferase on C-to-G and C-to-T mutations in Escherichia coli. Mutat. Res. 429:37-44
Abstract	The purpose of this study was to determine the effect of the Dcm cytosine methyltransferase on 5-azacytidine (5-azaC) mutagenesis in Escherichia coli. We used a Lac reversion assay to measure C-to-G and C-to-T mutations at a single, methylatable cytosine in the lacZ gene, in the presence and absence of Dcm. C-to-G mutations are stimulated by 5-azaC but are largely independent of Dcm. In contrast, C-to-T mutations are not stimulated by 5-azaC in either wild type or dcm cells. However, in cells which contain Dcm but are defective in very short patch repair, the normally high frequency of spontaneous C-to-T mutations is decreased by the analog in a dose-dependent manner.
Links	PubMed
Keywords	Azacitidine/pharmacology; DNA (Cytosine-5-)-Methyltransferase/genetics; DNA (Cytosine-5-)-Methyltransferase/metabolism; Dose-Response Relationship, Drug; Enzyme Inhibitors/pharmacology; Escherichia coli/drug effects; Escherichia coli/enzymology; Escherichia coli/genetics; Mutagenesis; Mutagenicity Tests; Mutation; Plasmids/genetics; Point Mutation/drug effects
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