PMID:10418149
| Citation |
Müller, JP (1999) Effects of pre-protein overexpression on SecB synthesis in Escherichia coli. FEMS Microbiol. Lett. 176:219-27 |
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| Abstract |
Protein translocation through the cytoplasmic membrane of Escherichia coli involves cytosolic chaperones. The export-dedicated chaperone SecB mediates targeting of a subset of pre-proteins. In this report, synthesis of SecB in response to plasmid-mediated overexpression of pre-proteins was studied. Overexpression of SecB-dependent pre-proteins stimulated synthesis of SecB under conditions where the cellular export capacity was saturated or uncomplexed SecB was trapped. On the contrary, overexpression of SecB-independent pre-beta-lactamase reduced the promoter activity of secB. The results suggest that uncomplexed SecB can be sequestered by synthesis of SecB-dependent pre-proteins. Furthermore, these data demonstrate the distinct action of the SecB- and signal recognition particle-dependent protein targeting pathways. |
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| Keywords |
ATP-Binding Cassette Transporters; Bacterial Outer Membrane Proteins/metabolism; Bacterial Proteins/biosynthesis; Bacterial Proteins/metabolism; Carrier Proteins/metabolism; Escherichia coli/metabolism; Escherichia coli Proteins; Maltose-Binding Proteins; Molecular Chaperones/metabolism; Monosaccharide Transport Proteins; Protein Precursors/metabolism; beta-Lactamases/metabolism |
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