PMID:10413098
| Citation |
Kulbachinskiy, A, Mustaev, A, Goldfarb, A and Nikiforov, V (1999) Interaction with free beta' subunit unmasks DNA-binding domain of RNA polymerase sigma subunit. FEBS Lett. 454:71-4 |
|---|---|
| Abstract |
The promoter recognition site on the sigma70 initiation factor is shielded from interaction with DNA unless sigma70 is bound to the core component of RNA polymerase (RNAP). It is shown that interaction of sigma70 with the isolated beta' subunit of Escherichia coli RNAP is sufficient to induce unshielding of the DNA binding site. Using UV-induced DNA-protein cross-linking we demonstrate that free beta' stimulates specific cross-links between region 2 of the sigma70 polypeptide and a fragment of the non-template promoter strand containing the TATAAT sequence. Thus the sigmabeta' subassembly of RNAP can assume a functionally competent conformation independently of the bulk of the RNAP core. |
| Links | |
| Keywords |
Cross-Linking Reagents; DNA-Directed RNA Polymerases/chemistry; DNA-Directed RNA Polymerases/metabolism; Dose-Response Relationship, Drug; Escherichia coli/enzymology; Models, Genetic; Protein Binding; Ultraviolet Rays |
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