PMID:10411911

Citation	Sääf, A, Johansson, M, Wallin, E and von Heijne, G (1999) Divergent evolution of membrane protein topology: the Escherichia coli RnfA and RnfE homologues. Proc. Natl. Acad. Sci. U.S.A. 96:8540-4
Abstract	Although the molecular evolution of protein tertiary structure and enzymatic activity has been studied for decades, little attention has been paid to the evolution of membrane protein topology. Here, we show that two closely related polytopic inner membrane proteins from Escherichia coli have evolved opposite orientations in the membrane, which apparently has been achieved by the selective redistribution of positively charged amino acids between the polar segments flanking the transmembrane stretches. This example of divergent evolution of membrane protein topology suggests that a complete inversion of membrane topology is possible with relatively few mutational changes even for proteins with multiple transmembrane segments.
Links	PubMed PMC17552
Keywords	Alkaline Phosphatase/genetics; Amino Acid Sequence; Bacterial Outer Membrane Proteins/chemistry; Cloning, Molecular; Electron Transport; Escherichia coli/chemistry; Evolution, Molecular; Membrane Proteins/chemistry; Membrane Proteins/genetics; Molecular Sequence Data; Mutation; Recombinant Fusion Proteins/genetics; Sequence Alignment
edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Add links to pages that link here (e.g. gene, product, method pages)

See Help:References for how to manage references in EcoliWiki.