PMID:10411265

Citation	Horswill, AR and Escalante-Semerena, JC (1999) The prpE gene of Salmonella typhimurium LT2 encodes propionyl-CoA synthetase. Microbiology (Reading, Engl.) 145 ( Pt 6):1381-8
Abstract	Biochemical and genetic evidence is presented to demonstrate that the prpE gene of Salmonella typhimurium encodes propionyl-CoA synthetase, an enzyme required for the catabolism of propionate in this bacterium. While prpE mutants used propionate as carbon and energy source, prpE mutants that lacked acetyl-CoA synthetase (encoded by acs) did not, indicating that Acs can compensate for the lack of PrpE in prpE mutants. Cell-free extracts enriched for PrpE catalysed the formation of propionyl-CoA in a propionate-, ATP-, Mg2+- and HS-CoA dependent manner. Acetate substituted for propionate in the reaction at 48% the rate of propionate; butyrate was not a substrate for PrpE. The propionyl-CoA synthetase activity of PrpE was specific for ATP. GTP, ITP, CTP and TTP were not used as substrates by the enzyme. UV-visible spectrophotometry, HPLC and MS data demonstrated that propionyl-CoA was the product of the reaction catalysed by PrpE.
Links	PubMed
Keywords	Chromatography, High Pressure Liquid; Coenzyme A Ligases/genetics; Coenzyme A Ligases/metabolism; Genes, Bacterial; Mass Spectrometry; Mutation; Operon; Repressor Proteins; Saccharomyces cerevisiae Proteins; Salmonella typhimurium/enzymology; Salmonella typhimurium/genetics
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