PMID:10403811

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Citation

Noji, H, Häsler, K, Junge, W, Kinosita, K Jr, Yoshida, M and Engelbrecht, S (1999) Rotation of Escherichia coli F(1)-ATPase. Biochem. Biophys. Res. Commun. 260:597-9

Abstract

By applying the same method used for F(1)-ATPase (TF(1)) from thermophilic Bacillus PS3 (Noji, H., Yasuda, R., Yoshida, M., and Kinosita, K., Jr. (1997) Nature 386, 299-302), we observed ATP-driven rotation of a fluorescent actin filament attached to the gamma subunit in Escherichia coli F(1)-ATPase. The torque value and the direction of the rotation were the same as those observed for TF(1). F(1)-ATPases seem to share common properties of rotation irrespective of the sources.

Links

PubMed Online version:10.1006/bbrc.1999.0885

Keywords

Actin Cytoskeleton/metabolism; Actins/metabolism; Adenosine Triphosphate/metabolism; Biotinylation; Escherichia coli/enzymology; Kinetics; Microscopy, Fluorescence; Mutation; Proton-Translocating ATPases/genetics; Proton-Translocating ATPases/metabolism; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Rotation; Streptavidin/metabolism; Torque

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