PMID:10403406
| Citation |
Mukhopadhyay, J, Sur, R and Parrack, P (1999) Functional roles of the two cyclic AMP-dependent forms of cyclic AMP receptor protein from Escherichia coli. FEBS Lett. 453:215-8 |
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| Abstract |
The cyclic AMP receptor protein activates transcription in Escherichia coli, only when complexed with cyclic AMP. The cyclic AMP receptor protein-cyclic AMP complex formed at low concentrations of cyclic AMP has a different conformation from either cyclic AMP receptor protein alone or its complex with cyclic AMP formed at high cyclic AMP concentrations. Various biophysical data suggest that the latter complex resembles free cyclic AMP receptor protein. We have examined the conformational and biological properties of cyclic AMP receptor protein as a function of cyclic AMP concentrations, using the gal operon of E. coli. A biphasic behavior is observed. It is shown that only the complex formed at lower concentrations of cyclic AMP is the transcriptionally active form. This difference between the complexes at different levels of cyclic AMP arises from a decreased ability of the cyclic AMP receptor protein-cyclic AMP complex at high cyclic AMP concentrations to bind to DNA at specific sites. |
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| Keywords |
Bacterial Proteins/chemistry; Bacterial Proteins/metabolism; Carrier Proteins; Cyclic AMP/pharmacology; Cyclic AMP Receptor Protein/chemistry; Cyclic AMP Receptor Protein/metabolism; DNA-Binding Proteins/chemistry; DNA-Binding Proteins/metabolism; Escherichia coli/genetics; Galactose/metabolism; Gene Expression Regulation, Bacterial; Operon; Protein Binding; Protein Conformation; Transcription, Genetic |
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