PMID:10398829
| Citation |
Bernstein, C, Bernstein, H, Payne, CM, Beard, SE and Schneider, J (1999) Bile salt activation of stress response promoters in Escherichia coli. Curr. Microbiol. 39:68-72 |
|---|---|
| Abstract |
Bile salts are prevalent in the mammalian intestine, a natural habitat of Escherichia coli. The bile salts deoxycholate, chenodeoxycholate, ursodeoxycholate, and glycocholate were tested for their effect on induction of 13 specific stress response genes. The most consistently activated E. coli promoters were those for genes micF, osmY, and dinD. MicF and osmY gene products are associated with membrane functions and are responsive to oxidative stress. DinD is induced by DNA damage as part of the SOS response. These results indicate that bile acids, to which E. coli are naturally exposed, induce expression of specific stress response genes, possibly in response to membrane perturbation, oxidative stress, and DNA damage. Altered expression of stress-response genes may also promote interaction of E. coli with cells of the colonic epithelium. |
| Links | |
| Keywords |
Bacterial Proteins/genetics; Bile Acids and Salts/pharmacology; Carrier Proteins/genetics; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Genes, Bacterial; Genes, Reporter; Heat-Shock Response/genetics; Humans; Periplasmic Binding Proteins; Promoter Regions, Genetic; Recombinant Fusion Proteins; beta-Galactosidase/genetics |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.