PMID:10395468
| Citation |
Chuang, SE and Blattner, FR (1999) Identification of phosphate-regulated genes by differential expression in the UV-irradiated host system. J. Microbiol. Methods 37:93-6 |
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| Abstract |
The UV-irradiated host system has been used for identifying protein products of genes cloned in a phage vector. By starving the host cells for phosphate immediately before UV-irradiation, we demonstrate that phosphate-regulated genes can be easily identified. By employing this new technique, we also provide evidence showing that the gpsA gene might be a new member of the phosphate starvation-inducible (psi) genes of E. coli. |
| Links | |
| Keywords |
Bacterial Proteins/genetics; Bacteriophage lambda; Culture Media/chemistry; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/radiation effects; Escherichia coli/virology; Gene Expression Regulation, Bacterial; Genes, Bacterial; Glycerolphosphate Dehydrogenase/genetics; Phosphates; Ultraviolet Rays |
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