PMID:10385000
| Citation |
Prost, JF and Cozzone, AJ (1999) Detection of an extended-10 element in the promoter region of the pckA gene encoding phosphoenolpyruvate carboxykinase in Escherichia coli. Biochimie 81:197-200 |
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| Abstract |
The regulation of transcription of the pckA gene coding for phosphoenolpyruvate carboxykinase in Escherichia coli was analysed by site-directed mutagenesis of the promoter region and measurement of in vitro transcription initiation. Mutation of the guanine residue at position -14 to either cytosine or thymine was found to result in a drastic decrease of transcription, even in the presence of the natural -35 hexamer TTTCCA that differs by only two nucleotides from the consensus sequence TTGACA. It was concluded that the promoter region of pckA contains an extended -10 module, 5'-TG-3', located one base upstream of the -10 hexamer, which is crucial for transcription. |
| Links | |
| Keywords |
Base Sequence; DNA, Bacterial; Escherichia coli/enzymology; Escherichia coli/genetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Phosphoenolpyruvate Carboxykinase (ATP)/genetics; Plasmids; Promoter Regions, Genetic; Transcription, Genetic |
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