PMID:10368163
Citation |
Murphy, LD, Rosner, JL, Zimmerman, SB and Esposito, D (1999) Identification of two new proteins in spermidine nucleoids isolated from Escherichia coli. J. Bacteriol. 181:3842-4 |
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Abstract |
The Escherichia coli nucleoid contains DNA in a condensed but functional form. Analysis of proteins released from isolated spermidine nucleoids after treatment with DNase I reveals significant amounts of two proteins not previously detected in wild-type E. coli. Partial amino-terminal sequencing has identified them as the products of rdgC and yejK. These proteins are strongly conserved in gram-negative bacteria, suggesting that they have important cellular roles. |
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Keywords |
Amino Acid Sequence; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/isolation & purification; Conserved Sequence; Deoxyribonuclease I; Escherichia coli/chemistry; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Gram-Negative Bacteria/chemistry; Molecular Sequence Data; Organelles/chemistry; Spermidine/metabolism |
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