PMID:10366529

Citation	Heinemann, JA (1999) Genetic evidence of protein transfer during bacterial conjugation. Plasmid 41:240-7
Abstract	DNA can be transferred from eubacteria to at least plants, fungi, and all other eubacteria by related, plasmid-mediated conjugation. Little is known about the biochemistry of intraspecies or interspecies DNA transfer. Even less is known about what other molecules may accompany the DNA, or the direct or inheritable effects on recipients of these escort molecules. This report describes a genetic assay for detecting protein transfer during conjugation. The assay monitored phage lambda released from lysogenic recipients as a result of the concomitant delivery of the Escherichia coli RecA protein and plasmid DNA. The heretofore unexpected transfer of a donor chromosome-encoded protein initiates a heritable change in the recipient without altering its genetic make-up. The mechanism of transfer could be independent of transferred DNA.
Links	PubMed Online version:10.1006/plas.1999.1392
Keywords	Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Bacteriophage lambda/genetics; Conjugation, Genetic; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli/virology; Gene Transfer Techniques; Genes, Bacterial; Lysogeny; Plasmids/genetics; Plasmids/metabolism; Rec A Recombinases/genetics; Rec A Recombinases/metabolism
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