PMID:10344250

Citation	Choe, LH, Chen, W and Lee, KH Proteome analysis of factor for inversion stimulation (Fis) overproduction in Escherichia coli. Electrophoresis 20:798-805
Abstract	The factor-for-inversion stimulation protein (Fis) is a global regulatory protein in Escherichia coli that activates ribosomal RNA (rRNA) transcription by binding to three upstream activation sites of the rRNA promoter and enhances transcription 5- to 10-fold in vivo. Fis overexpression results in different effects on cell growth depending on nutrient conditions. Differential proteome analysis of Fis-expressing cells shows ten protein spots corresponding to Fis overexpression in both rich (YT) and minimal (M9+glucose) media. Three of these spots have been identified as elongation factor TS, histidine-binding periplasmic protein precursor, and ketol-acid reductoisomerase.
Links	PubMed Online version:<798::AID-ELPS798>3.0.CO;2-F 10.1002/(SICI)1522-2683(19990101)20:4/5<798::AID-ELPS798>3.0.CO;2-F
Keywords	Bacterial Proteins/metabolism; Carrier Proteins/biosynthesis; Carrier Proteins/genetics; Culture Media; Electrophoresis, Gel, Two-Dimensional/methods; Escherichia coli/growth & development; Escherichia coli/metabolism; Escherichia coli Proteins; Factor For Inversion Stimulation Protein; Integration Host Factors; RNA-Binding Proteins/genetics; RNA-Binding Proteins/metabolism
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